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Drug Metabolism &
Pharmacokinetics (DMPK) Preclinical Discovery

TCGLS has more than 12 years of experience in providing DMPK and bioanalytical services to global pharmaceutical, biotech, animal health companies supporting their preclinical drug discovery programs. Our state-of-the art DMPK facility is well equipped with multiple liquid handling & LC-MS systems as well as in-house breeding facility to maintain regular supply of rodents for experiments.

Our team of highly skilled and dedicated scientists has extensive experience in broad spectrum of DMPK studies to add value to our customers’ discovery efforts by providing reliable data in an economic and efficient manner. We offer flexible solutions by developing customized assays as and when required to rapidly adapt to the project needs.

in Vitro ADME (small molecule and peptides)

  • Physicochemical screening: Kinetic & Thermodynamic solubility, LogD
  • Solubility in biorelevant media (FaSSIF, FeSSIF, SGF)
  • Chemical stability in buffer and biorelevant media
  • Absorption/Permeability: PAMPA, Caco-2, MDCK-II, MDCK-MDR1
  • Efflux Transporter assay (Substrate/Inhibitor screens): P-gp, BCRP, BSEP, MRP2, MRP4
  • Uptake Transporter assay (Substrate/Inhibitor screens)*: OATP1B1, OATP1B3
  • Hepatocytes uptake assay
  • Metabolic stability: S9, microsomes & hepatocytes
  • AO/UGT based metabolism
  • Drug-drug interaction: CYP inhibition (multiple CYPs)
  • Drug-drug interaction: CYP induction (CYP1A2, 2B6, 3A4)
  • Time dependent drug-drug interaction (TDI)
  • Plasma stability
  • Plasma Protein Binding
  • Microsomal & hepatocellular binding
  • Brain tissue binding
  • Blood to plasma ratio
  • Metabolite ID (Soft spot analysis)
  • CYP450 reaction phenotyping

In Vivo Pharmacokinetics (Rats/Mice)

  • Discovery formulation development
  • Bioavailability study 
  • PK Profiling through multiple routes (PO, IP, IV, IM, SC, ID, IT etc.)
  • Preclinical bioequivalence study
  • Single/multiple dose PK 
  • Cassette (5-in-one) PK 
  • Tissue/ CSF distribution study
  • CNS penetration study
  • Drug administration through continuous IV infusion (mechanical /osmotic pump )
  • Non-compartmental pharmacokinetic analysis, WinNonLin
  • Metabolite quantitation

In Vivo Toxicokinetics

  • Formulation optimization with exposure evaluation
  • LC-MS/MS bioanalysis of TK samples

Discovery Bioanalysis

  • Non-GLP LC-MS/MS bioanalytical support
  • Bioanalysis of biofluids – Plasma, blood, CSF, urine, bile, synovial fluid etc.
  • Bioanalysis of tissues – Brain, liver, spleen, lung, muscle, paw, fat tissue etc.
  • Small molecule Biomarker analysis
  • Peptide drug analysis

List of state of the art equipment>>