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Molecular Pharmacology Preclinical Discovery

Cellular and Molecular Biology

TCGLS boasts of having more than 15 years of experience in developing innovative assays and screening methods to evaluate effects of chemical compounds on cellular, molecular and biochemical processes. The screens, further help to unravel cellular functions/biological process that could lead to identification of potential therapeutic candidates.

Expertise includes:

  • Molecular cloning, SDM,lab-scale protein expression and affinity based purification
  • Cell line development services in line with assay requirements (validation and optimization included)
  • Diverse range of in vitro assays (primary, secondary and tertiary) optimized and validated in multiple therapeutic areas
  • Primary cell-based specialized assays across therapeutic areas
  • Adopting screening assays from bench scale assays to automation in 96 and 384 plate formats
  • Tech transfer assays effectively implemented
  • Statistical analysis of the data sacrosanctly satisfy pre-defined acceptance criteria

Therapeutic Areas

  • Cardiovascular & Metabolic Disorders
  • Inflammation and pain
  • CNS
  • Oncology
  • Anti-infectives (anti-bacterial & anti-fungal)

Targets

  • GPCR
  • Ion channel
  • Enzyme
  • NHR
  • Transporter

List of state of the art equipment>>

Platforms & Assay Technologies

  • Alphascreen
  • Beta arrestin
  • cAMP
  • ELISA (Classical/HTRF)
  • Enzyme assays: Fluorescence & radio-isotope labeled
  • Fluorescence Polarization
  • GTPgamma binding
  • HTRF and 
  • TR-FRET
  • Intracellular calcium mobilization
  • LCMS based assay
  • Mechanistic Characterization: Competitive, non-competitive & Allosteric
  • Radioligand binding (SPA & filtration)
  • Thallium flux
  • Ultraluminescence
  • Voltage sensitive dye assays
  • Voltage Sensor Probe-based FRET assay
  • Expression Profiling mRNA and Protein
  • Thermal Shift Assay
  • Protein degradation